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国际木文化学会 > 主题 > 学术研究 > 研讨会 > 2022世界木材日研讨会 > Phytochemical and In vitro Antioxidant and Antimicrobial Activity of Indigenous Vegetables of Ilocos Norte
Phytochemical and In vitro Antioxidant and Antimicrobial Activity of Indigenous Vegetables of Ilocos Norte
国际木文化学会 | 37 view(s) | 2022/09/08

会议名称:“2022世界木材日”研讨会暨第四届国际林联(IUFRO)林产品文化研究组讨论会
会议时间:2022年3月23日

报告嘉宾:Maingelline B. Vivit
嘉宾简介:菲律宾马里亚诺马科斯国立大学 二级助理研究员


摘要:

Indigenous plants are excellent sources of vitamins, minerals, and phytochemicals. However, sample processing affects the phytoconstituents and health benefits of plants. In this study, the antioxidant activity of the raw, boiled, steamed, and dried Broussonetia luzonica, Champereia manillana, Telosma procumbens, Momordica cochinchinensis; Tetrastigma harmandii, Solanum impinellifolium, Poikilospermum suaveolens, and Dioscorea esculenta was determined thru 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The antimicrobial activity was tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, and Candida albicans. Moreover, total phenolics (TPC) and flavonoids content (TFC) of the indigenous plant extracts were assessed using Folin-Ciocalteu and Aluminum chloride method, respectively, to determine the corresponding effect on the biological activities of the indigenous plants. 

TPC and TFC of the raw, boiled, steamed, and dried IVs ranged from 3.60 to 273.97, 4.50-188.52, 4.40-149.66; and 3.44-63.22, 4.89-63.85, 0-61.47, 4.06-164.75µg/mL in gallic acid and quercetin equivalents, respectively.  Radical scavenging activity ranged from 0-90.11, 0.60-88.81, 0-91.49 and 0-93.19%. The effect of the preparation methods on TPC, TFC, and antioxidant activity was significant (p < 0.01) in all the indigenous vegetables. However, the increase or decrease in the amount depends on the plant species. Moreover, correlation analysis showed that the TPC and TFC are not related to the antioxidant activity. Furthermore, steamed C. manillana leaf tops showed high activity against S. aureus while mild activity against S. aureus and M. luteus was observed using the dried extract. Thus, results show that indigenous vegetables are healthy and could be a possible source of functional foods. The preparation method would also serve as the basis to obtain high antioxidants and possess antibacterial activity.


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